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Rnaseh2btm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Rnaseh2btm1a(EUCOMM)Wtsi
Name: ribonuclease H2, subunit B; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432164
Synonyms: Rnaseh2btm1a, RNaseH2null
Gene: Rnaseh2b  Location: Chr14:62292589-62372992 bp, + strand  Genetic Position: Chr14, 33.19 cM, cytoband C3
Mutation
origin
Mutant Cell Lines:  EPD0087_4_A01, EPD0087_4_A02, EPD0087_4_A03, EPD0087_4_A04, EPD0087_4_B01, EPD0087_4_C01, EPD0087_4_C02, EPD0087_4_C04, EPD0087_4_D03, EPD0087_4_D04, EPD0087_4_E03, EPD0087_4_F03, EPD0087_4_G01, EPD0087_4_G03, EPD0087_4_H01, EPD0087_4_H02, EPD0087_4_H03, EPD0087_4_H04 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:186180
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 62353057 of Chromosome 14 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 62353921. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 7 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rnaseh2b Mutation:  33 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory