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P2rx7tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: P2rx7tm1a(EUCOMM)Wtsi
Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432150
Gene: P2rx7  Location: Chr5:122643911-122691432 bp, + strand  Genetic Position: Chr5, 62.5 cM, cytoband F
Mutation
origin
Mutant Cell Lines:  EPD0369_6_A05, EPD0369_6_A06, EPD0369_6_A07, EPD0369_6_A08, EPD0369_6_B06, EPD0369_6_B07, EPD0369_6_B08, EPD0369_6_C05, EPD0369_6_C06, EPD0369_6_C08, EPD0369_6_D05, EPD0369_6_D07, EPD0369_6_D08, EPD0369_6_E06, EPD0369_6_E07, EPD0369_6_F05, EPD0369_6_F07, EPD0369_6_G05, EPD0369_6_G06, EPD0369_6_G07, EPD0369_6_G08, EPD0369_6_H05, EPD0369_6_H06, EPD0369_6_H07, EPD0369_6_H08
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 122652263 of Chromosome 5 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 122653166. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any P2rx7 Mutation:  15 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory