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Rhot1tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Rhot1tm1a(EUCOMM)Wtsi
Name: ras homolog family member T1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432006
Synonyms: Miro1KO
Gene: Rhot1  Location: Chr11:80209019-80267907 bp, + strand  Genetic Position: Chr11, 47.62 cM, cytoband B5
Mutation
origin
Mutant Cell Lines:  EPD0066_2_A01, EPD0066_2_A02, EPD0066_2_A03, EPD0066_2_B01, EPD0066_2_B02, EPD0066_2_B03, EPD0066_2_C01, EPD0066_2_C02, EPD0066_2_C03, EPD0066_2_D01, EPD0066_2_D03, EPD0066_2_E01, EPD0066_2_E02, EPD0066_2_F01, EPD0066_2_F02, EPD0066_2_F03, EPD0066_2_G01, EPD0066_2_G02, EPD0066_2_G03, EPD0066_2_H01, EPD0066_2_H02, EPD0066_2_H03
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt1
 
Mutation detailsThe L1L2_gt1 cassette was inserted at position 80219721 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 80220746. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Rhot1 Mutation:  6 strains or lines available
Notes
Cell line EPD0066_2_F01 was successfully used to make chimeric mice. Germline transmission was accomplished. J:240500
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/19/2020
MGI 6.15
The Jackson Laboratory