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Targeted Allele Detail
Symbol: Cap2tm1a(EUCOMM)Wtsi
Name: CAP, adenylate cyclase-associated protein, 2 (yeast); targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4431970
Synonyms: cap2-
Gene: Cap2  Location: Chr13:46501848-46649642 bp, + strand  Genetic Position: Chr13, 23.83 cM
Mutant Cell Lines:  EPD0155_4_B07, EPD0155_4_C07, EPD0155_4_C08, EPD0155_4_D07, EPD0155_4_D08, EPD0155_4_E05, EPD0155_4_E06, EPD0155_4_E07, EPD0155_4_G07, EPD0155_4_G08, EPD0155_4_H06 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 46530525 of Chromosome 13 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 46531367. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:155845, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cap2 Mutation:  29 strains or lines available
Cell lines EPD0155_4_C08 and EPD0155_4_E05 were successfully used to make chimeric mice. Germline transmission was accomplished. J:234044
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.08
The Jackson Laboratory