About   Help   FAQ
Vps33btm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Vps33btm1a(EUCOMM)Wtsi
Name: vacuolar protein sorting 33B; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4431968
Gene: Vps33b  Location: Chr7:79919369-79941327 bp, + strand  Genetic Position: Chr7, 45.59 cM
Mutation
origin
Mutant Cell Lines:  EPD0339_4_H06, EPD0339_4_H07, EPD0339_5_D06, EPD0339_5_D07, EPD0339_5_E05, EPD0339_5_E06, EPD0339_5_E08, EPD0339_5_F05, EPD0339_5_F06, EPD0339_5_G08, EPD0339_5_H06, EPD0339_5_H08
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 79923527 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 79924655. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Vps33b Mutation:  19 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/23/2021
MGI 6.17
The Jackson Laboratory