Cpa1^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Cpa1^{tm1a(KOMP)Mbp}
• Name: carboxypeptidase A1, pancreatic; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4431517
• Gene: Cpa1 Location: Chr6:30639217-30645360 bp, + strand Genetic Position: Chr6, 12.52 cM, cytoband B1
• IMPC: Cpa1 gene page

Mutation origin

• Mutant Cell Lines: DEPD0001_2_A09, DEPD0001_2_A10, DEPD0001_2_B09, DEPD0001_2_B10, DEPD0001_2_C09, DEPD0001_2_C10, DEPD0001_2_C12, DEPD0001_2_D12, DEPD0001_2_E09, DEPD0001_2_E10, DEPD0001_2_E11, DEPD0001_2_E12, DEPD0001_2_F09, DEPD0001_2_F10, DEPD0001_2_F12, DEPD0001_2_G09, DEPD0001_2_H09, DEPD0001_2_H10, DEPD0001_2_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30640329 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30642547. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpa1 Mutation: 25 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)