Tg(tetO-GCaMP2)12JRyu
Transgene Detail

Summary

• Symbol: Tg(tetO-GCaMP2)12JRyu
• Name: transgene insertion 12J, Ron Yu
• MGI ID: MGI:4431217
• Synonyms: tetO-G-CaMP2
• Transgene: Tg(tetO-GCaMP2)12JRyu Location: unknown
• Alliance: Tg(tetO-GCaMP2)12JRyu page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: This transgene contains an unspecified promoter followed by seven copies of the tetracycline responsive operator (tetO), cDNA encoding the Ca²⁺ reporter GCaMP2, and a polyadenylation signal. GCaMP2 is a circularly permuted enhanced green fluorescent protein (EGFP) interrupted at amino acid (aa) 145 so that the N-terminal 144 aa and the C-terminal 90 aa have exchanged places; calmodulin (CaM) and a 13 aa peptide of myosin light chain kinase (M13) have been appended to the new C- and N-termini, respectively. The 35-aa polyHis plasmid leader sequence RSET, found to be essential for thermal stability, resides N-terminal to M13, and mutations have been introduced to improve fluorescence (Asp180Tyr and Val93Ile) and thermal stability (Val163Aln and Ser175Gly) and to prevent GFP dimerization (Ala206Lys). Fluorescence of GCaMP2 is induced by interaction of the C-terminal calmodulin, in association with Ca²⁺, with the N-terminal M13. (J:107637, J:157448)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:157448 He J, et al., Encoding gender and individual information in the mouse vomeronasal organ. Science. 2008 Apr 25;320(5875):535-8
• All: 6 reference(s)