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Lyve1tm1.1(EGFP/cre)Cys
Targeted Allele Detail
Nomenclature
Symbol: Lyve1tm1.1(EGFP/cre)Cys
Name: lymphatic vessel endothelial hyaluronan receptor 1; targeted mutation 1, Jason G Cyster
MGI ID: MGI:4421655
Synonyms: Lyve-1 Cre+, Lyve-1 EGFP-hCre
Gene: Lyve1  Location: Chr7:110850607-110862953 bp, - strand  Genetic Position: Chr7, 57.89 cM, cytoband F2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:156826
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsAn EGFP fused to a humanized cre (hCre) sequence and preceded by an internal ribosomal entry site (IRES) was inserted into the 3' UTR of the Lyve1 locus by homologous recombination in E14 ES cells. This construct was followed by an FRT-flanked neo selection cassette. Three successfully targeted clones were injected into C57BL/6 blastocysts and chimeras derived from clone 9 were bred to get germline transmission. The neo cassette was deleted in the germline with Flp recombinase. Selective GFP expression in nuclei of Lyve1 +ve cells is detected by antibody staining. (J:156826)
Generation of the Lyve1tm1.1(EGFP/cre)Cys allele
Recombinase
activity
Activity:
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Driver: Lyve1     Summary of all recombinase alleles driven by Lyve1. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lyve1 Mutation:  9 strains or lines available
References
Original:  J:156826 Pham TH, et al., Lymphatic endothelial cell sphingosine kinase activity is required for lymphocyte egress and lymphatic patterning. J Exp Med. 2010 Jan 18;207(1):17-27, S1-4
All:  29 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory