Lyve1tm1.1(EGFP/cre)Cys
Targeted Allele Detail
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Symbol: |
Lyve1tm1.1(EGFP/cre)Cys |
Name: |
lymphatic vessel endothelial hyaluronan receptor 1; targeted mutation 1, Jason G Cyster |
MGI ID: |
MGI:4421655 |
Synonyms: |
Lyve-1 Cre+, Lyve-1 EGFP-hCre |
Gene: |
Lyve1 Location: Chr7:110449814-110462160 bp, - strand Genetic Position: Chr7, 57.89 cM, cytoband F2
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Alliance: |
Lyve1tm1.1(EGFP/cre)Cys page
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Germline Transmission: |
Earliest citation of germline transmission:
J:156826
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Parent Cell Line: |
E14 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Lyve1tm1.1(EGFP/cre)Cys expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: An EGFP fused to a humanized cre (hCre) sequence and preceded by an internal ribosomal entry site (IRES) was inserted into the 3' UTR of the Lyve1 locus by homologous recombination in E14 ES cells. This construct was followed by an FRT-flanked neo selection cassette. Three successfully targeted clones were injected into C57BL/6 blastocysts and chimeras derived from clone 9 were bred to get germline transmission. The neo cassette was deleted in the germline with Flp recombinase. Selective GFP expression in nuclei of Lyve1 +ve cells is detected by antibody staining.
(J:156826)
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Generation of the Lyve1tm1.1(EGFP/cre)Cys allele |
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Lyve1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:156826 Pham TH, et al., Lymphatic endothelial cell sphingosine kinase activity is required for lymphocyte egress and lymphatic patterning. J Exp Med. 2010 Jan 18;207(1):17-27, S1-4 |
All: |
62 reference(s) |
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