Zfpm1^tm1.1Esv
Targeted Allele Detail

Summary

• Symbol: Zfpm1^tm1.1Esv
• Name: zinc finger protein, multitype 1; targeted mutation 1.1, Eric C Svensson
• MGI ID: MGI:4421436
• Synonyms: FOG-1^R3K5A
• Gene: Zfpm1 Location: Chr8:123008880-123063990 bp, + strand Genetic Position: Chr8, 70.86 cM, cytoband E1
• Alliance: Zfpm1^tm1.1Esv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156475
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A cDNA encoding two point mutations in the FOG repression motif (asparagine 3 to alanine and lysine 5 to alanine), which disrupt the interaction of the protein with subunits of the nucleosome remodeling and histone deacetylase complex, and a floxed neo cassette within intron 1, was inserted by homologous recombination. The neo cassette was removed by cre-mediated recombination. Western blot analysis in bone marrow cell lysates from homozygous mice confirmed expression of the mutant protein at levels similar to those of the wild-type protein. (J:156475)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfpm1 Mutation: 31 strains or lines available

Notes

AA- p.Asn3Ala, p.Lys5Ala

References

• Original: J:156475 Gao Z, et al., FOG-1-mediated recruitment of NuRD is required for cell lineage re-enforcement during haematopoiesis. EMBO J. 2010 Jan 20;29(2):457-68
• All: 1 reference(s)