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Hoga1tm2a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Hoga1tm2a(KOMP)Wtsi
Name: 4-hydroxy-2-oxoglutarate aldolase 1; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID: MGI:4419886
Synonyms: Hoga1-
Gene: Hoga1  Location: Chr19:42045610-42070953 bp, + strand  Genetic Position: Chr19, 35.67 cM, cytoband D1
Mutation
origin
Mutant Cell Lines:  EPD0380_4_A05, EPD0380_4_B06, EPD0380_4_B07, EPD0380_4_B08, EPD0380_4_C08, EPD0380_4_D06, EPD0380_4_E05, EPD0380_4_E08, EPD0380_4_G06, EPD0380_4_H08
Germline Transmission:  Earliest citation of germline transmission: J:231386
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 42059455 of Chromosome 19 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 42060455. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Hoga1 Mutation:  14 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/14/2020
MGI 6.14
The Jackson Laboratory