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Myo18atm1a(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Myo18atm1a(KOMP)Wtsi
Name: myosin XVIIIA; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4419827
Gene: Myo18a  Location: Chr11:77654072-77756806 bp, + strand  Genetic Position: Chr11, 46.74 cM
Alliance: Myo18atm1a(KOMP)Wtsi page
IMPC: Myo18a gene page
Mutation
origin
Mutant Cell Lines:  EPD0396_4_C05, EPD0396_4_C07, EPD0396_4_D07, EPD0396_4_E05, EPD0396_4_E07, EPD0396_4_F05, EPD0396_4_F06, EPD0396_4_F08, EPD0396_4_G08, EPD0396_4_H06
Germline Transmission:  Earliest citation of germline transmission: J:277320
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 77710414 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77712192. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Myo18a Mutation:  97 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/22/2022
MGI 6.22
The Jackson Laboratory