Bmf^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bmf^{tm1(KOMP)Wtsi}
• Name: BCL2 modifying factor; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419589
• Gene: Bmf Location: Chr2:118359238-118380168 bp, - strand Genetic Position: Chr2, 59.4 cM, cytoband E5
• IMPC: Bmf gene page

Mutation origin

• Mutant Cell Lines: EPD0391_5_A03, EPD0391_5_B02, EPD0391_5_B04, EPD0391_5_C02, EPD0391_5_C04, EPD0391_5_D01, EPD0391_5_F04, EPD0391_5_G03, EPD0391_5_G04, EPD0391_5_H03
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4190 starting at position 118375495 and ending at position 118379685 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Bmf Mutation: 23 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)