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2210016L21Riktm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: 2210016L21Riktm1a(KOMP)Wtsi
Name: RIKEN cDNA 2210016L21 gene; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4419127
Gene: 2210016L21Rik  Location: Chr5:114942154-114948540 bp, + strand  Genetic Position: Chr5, 55.99 cM, cytoband F
Mutation
origin
Mutant Cell Lines:  EPD0365_4_A09, EPD0365_4_A10, EPD0365_4_A12, EPD0365_4_C09, EPD0365_4_C12, EPD0365_4_D09, EPD0365_4_E09, EPD0365_4_E10, EPD0365_4_E12, EPD0365_4_F10, EPD0365_4_F12, EPD0365_4_G11, EPD0365_4_G12, EPD0365_4_H09, EPD0365_4_H10
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 114945295 of Chromosome 5 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 114947507. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 2 lines available
Carrying any 2210016L21Rik Mutation:  11 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory