Tmod4^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmod4^{tm1(KOMP)Wtsi}
• Name: tropomodulin 4; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419118
• Synonyms: Tmod4^-
• Gene: Tmod4 Location: Chr3:95031787-95036520 bp, + strand Genetic Position: Chr3, 40.74 cM
• Alliance: Tmod4^{tm1(KOMP)Wtsi} page
• IMPC: Tmod4 gene page

Mutation origin

• Mutant Cell Lines: EPD0400_4_B05, EPD0400_4_B06, EPD0400_4_B07, EPD0400_4_B08, EPD0400_4_C05, EPD0400_4_C06, EPD0400_4_C07, EPD0400_4_C08, EPD0400_4_D05, EPD0400_4_D06, EPD0400_4_D07, EPD0400_4_D08, EPD0400_4_E05, EPD0400_4_E06, EPD0400_4_E07, EPD0400_4_E08, EPD0400_4_F05, EPD0400_4_F06, EPD0400_4_F07, EPD0400_4_G06, EPD0400_4_G08, EPD0400_4_H05, EPD0400_4_H06, EPD0400_4_H07
• Germline Transmission: Earliest citation of germline transmission: J:236433
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2999 starting at position 95033123 and ending at position 95036122 of Chromosome 3 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534, J:236433)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Tmod4 Mutation: 13 strains or lines available

Notes

RNAi depletion of Tmod1 from either wild-type or Tmod4^{tm1(KOMP)Wtsi} homozygous mutant muscle fibers leads to thin filament elongation by ~15%, indicating that Tmod1 per se, rather than total sarcomeric Tmod levels, regulates thin filament length in mouse skeletal muscle (J:236433).

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)