Tmem178^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem178^{tm1(KOMP)Wtsi}
• Name: transmembrane protein 178; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419108
• Gene: Tmem178 Location: Chr17:81252061-81309245 bp, + strand Genetic Position: Chr17, 50.97 cM, cytoband E3
• Alliance: Tmem178^{tm1(KOMP)Wtsi} page
• IMPC: Tmem178 gene page

Mutation origin

• Mutant Cell Lines: EPD0407_4_C04, EPD0407_4_E01, EPD0407_4_E03
• Germline Transmission: Earliest citation of germline transmission: J:287421
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 3715 starting at position 81293595 and ending at position 81297310 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Tmem178 Mutation: 13 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)