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Transgene Detail
Symbol: Tg(Col2a1-rtTA,tetO-cre)22Pjro
Name: transgene insertion 22, Peter J Roughley
MGI ID: MGI:4418267
Synonyms: COL2-rtTA-Cre, Tg(Col2a1-rtTA-tetO-cre)22Pjro
Transgene: Tg(Col2a1-rtTA,tetO-cre)22Pjro  Location: unknown  
Strain of Origin:  Not Specified
Transgene Type:    Transgenic (Inducible, Recombinase, Transactivator)
Inducer:    doxycycline
Mutation:    Insertion
Mutation detailsThe construct was generated such that a 6 kb fragment of the Col2a1 gene (composed of 3 kb of promoter and 3 kb of intron 1 containing an enhancer sequence) controls the reverse tetracycline transactivator sequence. A tetracycline responsive element heptad sequence (tetO) with a CMV minimal promoter is upstream of the cre recombinase coding sequence in this construct. Cre expression can only occur by transactivation of rtTA by doxycycline. The linearized construct was injected into fertilized oocytes and founder mice were obtained (lines 13, 12, 22, and 24). Cre mRNA expression was detected only in cartilage, not in the brain, liver or skin of embryos exposed to doxycycline. (J:107728)
 Tissue activity of this recombinase allele
Driver: Col2a1     Summary of all recombinase alleles driven by Col2a1.

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Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:107728 Grover J, et al., Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biol. 2006 Apr;25(3):158-65
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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