Prnp^tm1Lnq
Targeted Allele Detail

Summary

• Symbol: Prnp^tm1Lnq
• Name: prion protein; targeted mutation 1, Susan Lindquist
• MGI ID: MGI:4412098
• Synonyms: PrP^3F4WT
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^tm1Lnq page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154937
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The third exon was replaced with an HPRT mini gene that was then replaced with a representative sequence containing two amino acid substitutions found in the human sequence (L108M and V111M). These substitutions allow for transmission barrier against mouse scrapies and immunoreactivity to the 3F4 antibody. (J:154937)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 150 strains or lines available

References

• Original: J:154937 Jackson WS, et al., Spontaneous generation of prion infectivity in fatal familial insomnia knockin mice. Neuron. 2009 Aug 27;63(4):438-50
• All: 2 reference(s)