Nmnat2^{Gt(EUCE0262a08)Hmgu}
Gene trapped Allele Detail

Summary

• Symbol: Nmnat2^{Gt(EUCE0262a08)Hmgu}
• Name: nicotinamide nucleotide adenylyltransferase 2; gene trap EUCE0262a08, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4371844
• Synonyms: Nmnat2^gt, Nmnat2^gtE
• Gene: Nmnat2 Location: Chr1:152830744-152995007 bp, + strand Genetic Position: Chr1, 65.15 cM
• Alliance: Nmnat2^{Gt(EUCE0262a08)Hmgu} page

Hunched posture, failure to initiate respiration, and swollen bladders in E18.5 Nmnat2^{Gt(EUCE0262a08)Hmgu}/Nmnat2^{Gt(RRF238)Byg} pups

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EUCE0262a08
• Germline Transmission: Earliest citation of germline transmission: J:192196
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: rsFlipROSAbgeo*
• Mutation details: The gene trap cassette is flanked with FRT, F3, loxP and lox511 sites. The sites are oriented to allow for inversion of the gene trap cassette. The gene trap construct inserted into intron 1. Immunoblot analysis confirmed the absence of protein expression in brains from homozygous embryos at E13-E14.5, but a trace amount of mRNA was detected by RT-PCR. (J:200901)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  EUCE0262a08.q1k5SPK	FI555264 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr1:152886494-152886907 bp (+)
  EUCE0262a08.p1k3SPK	FI541332 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr1:152886993-152887174 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  206 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nmnat2 Mutation: 270 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 8 reference(s)