Tg(Slc6a4-cre/ERT2)EZ8Gsat
Transgene Detail

Summary

• Symbol: Tg(Slc6a4-cre/ERT2)EZ8Gsat
• Name: transgene insertion EZ8, GENSAT Project at Rockefeller University
• MGI ID: MGI:4367236
• Synonyms: Founder Line EZ8, Tg(Slc6a4-cre/Esr1)8Gsat
• Transgene: Tg(Slc6a4-cre/ERT2)EZ8Gsat Location: unknown
• Alliance: Tg(Slc6a4-cre/ERT2)EZ8Gsat page

Transgene origin

• Strain of Origin: FVB/N
• Project Collection: GENSAT

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Slc6a4-cre/ERT2)EZ8Gsat expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc6a4 (MGI:96285)

• Mutation details: This transgene contains the coding sequence for the Cre-ERT2 fusion protein, followed by a polyadenylation signal, inserted into a mouse genomic artificial chromosome (BAC) at the ATG transcription initiation codon of the solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 gene so that expression of the tamoxifen-activated recombinase is driven by the regulatory sequences of the mouse gene. ERT2 is a triply mutated (G400V, M543A, L544A) human estrogen receptor ligand-binding domain that renders the fusion protein highly sensitive to activation by 4-hydroxytamoxifen, but unresponsive to the receptor's native ligands. (J:100256, J:145110)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a4 (mouse) Summary of all recombinase alleles driven by Slc6a4. Comparative matrix view of recombinase activities Alliance mouse Slc6a4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:100256 The Gene Expression Nervous System Atlas (GENSAT) Project, The Rockefeller University (New York, NY), MGI download of GENSAT transgene data. Database Download. 2005
• All: 2 reference(s)