Tg(Vil1-MYC/ErT)4Gev
Transgene Detail

Summary

• Symbol: Tg(Vil1-MYC/ErT)4Gev
• Name: transgene insertion 4, Gerard Evan
• MGI ID: MGI:4366334
• Synonyms: Tg(Vil-MYC/Er)4Gev, villin-MycER^TAM
• Transgene: Tg(Vil1-MYC/ErT)4Gev Location: unknown
• Alliance: Tg(Vil1-MYC/ErT)4Gev page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: not specified
• Mutation: Insertion
• Mutation details: A tissue-specific transgene encoding an inducible MYC protein was generated by coupling the mouse villin promoter (including 3.5 kb upstream of the transcription start site and 5.5 kb of the first intron) to a fusion cDNA encoding the human MYC protein joined to a modified mouse estrogen receptor (ER) ligand-binding domain. The Gly525Arg mutant ER domain (ER^TM) responds to 4-hydroxytamoxifen (4OHT) but is insensitive to the endogenous ligand, 17beta-estradiol; therefore, MYC is active only in the presence of 4OHT. Immunofluorescence microscopic analysis confirmed the expression of the fusion protein throughout the intestinal epithelium of transgenic mice. (J:53387, J:118587, J:153653)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:153653 Finch AJ, et al., Acute overexpression of Myc in intestinal epithelium recapitulates some but not all the changes elicited by Wnt/beta-catenin pathway activation. Mol Cell Biol. 2009 Oct;29(19):5306-15
• All: 4 reference(s)