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Ampd3tm2a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ampd3tm2a(KOMP)Wtsi
Name: adenosine monophosphate deaminase 3; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID: MGI:4364610
Gene: Ampd3  Location: Chr7:110768206-110812405 bp, + strand  Genetic Position: Chr7, 57.85 cM, cytoband E2-E3
Mutation
origin
Mutant Cell Lines:  EPD0043_3_B02, EPD0043_3_G03 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt2
 
Mutation detailsThe L1L2_gt2 cassette was inserted at position 110788159 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 110789075. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:148605, J:173534, J:206035)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ampd3 Mutation:  16 strains or lines available
Notes
Cell line EPD0043_3_B02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200072 J:206035
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/14/2017
MGI 6.08
The Jackson Laboratory