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Sel1ltm1e(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Sel1ltm1e(KOMP)Wtsi
Name: sel-1 suppressor of lin-12-like (C. elegans); targeted mutation 1e, Wellcome Trust Sanger Institute
MGI ID: MGI:4364219
Gene: Sel1l  Location: Chr12:91806043-91849157 bp, - strand  Genetic Position: Chr12, 45.02 cM, cytoband E
Mutation
origin
Mutant Cell Lines:  EPD0284_3_D08, EPD0284_3_F05
Germline Transmission:  Earliest citation of germline transmission: J:211773
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 91831199 of Chromosome 12 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Sel1l Mutation:  2 strains or lines available
Notes

Conflicting reports exist for the integrity of the ES mutant cell line EPD0284_3_D08. A published report (J:206568) describes the line as carrying the Sel1ltm1a(KOMP)Wtsi allele and the Sel1ltm1c(KOMP)Wtsi allele derived from it. However, analysis at the Wellcome Trust Sanger Institute suggests that this ES cell line only carries the Sel1ltm1e(KOMP)Wtsi allele.

References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory