About   Help   FAQ
Rtf1tm1a(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Rtf1tm1a(KOMP)Wtsi
Name: RTF1, Paf1/RNA polymerase II complex component; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4363925
Gene: Rtf1  Location: Chr2:119505549-119565888 bp, + strand  Genetic Position: Chr2, 59.97 cM, cytoband E5
Alliance: Rtf1tm1a(KOMP)Wtsi page
IMPC: Rtf1 gene page
Mutation
origin
Mutant Cell Lines:  EPD0033_5_C05, EPD0033_5_D05, EPD0033_5_E04, EPD0033_5_E05, EPD0033_5_F05, EPD0033_5_G05, EPD0033_5_H04, EPD0033_5_H05
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 119531110 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 119531977. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Rtf1 Mutation:  52 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory