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Ddx27tm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ddx27tm1a(KOMP)Wtsi
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4363861
Gene: Ddx27  Location: Chr2:167015193-167034947 bp, + strand  Genetic Position: Chr2, 87.22 cM
Mutation
origin
Mutant Cell Lines:  EPD0033_1_A02, EPD0033_1_B01, EPD0033_1_B02, EPD0033_1_C01, EPD0033_1_C02, EPD0033_1_D01, EPD0033_1_D02, EPD0033_1_E01, EPD0033_1_E02, EPD0033_1_F02, EPD0033_1_G01, EPD0033_1_H01, EPD0033_1_H02 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 167021625 of Chromosome 2 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 167022420. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:148605, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ddx27 Mutation:  19 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory