Cntd1^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cntd1^{tm1e(KOMP)Wtsi}
• Name: cyclin N-terminal domain containing 1; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363686
• Gene: Cntd1 Location: Chr11:101170029-101179527 bp, + strand Genetic Position: Chr11, 64.56 cM
• Alliance: Cntd1^{tm1e(KOMP)Wtsi} page
• IMPC: Cntd1 gene page

Mutation origin

• Mutant Cell Lines: EPD0190_3_A01, EPD0190_3_B01, EPD0190_3_B03, EPD0190_3_D02, EPD0190_3_E03, EPD0190_3_E04, EPD0190_3_G01, EPD0190_3_H01
• Germline Transmission: Earliest citation of germline transmission: J:215796
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101173775 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cntd1 Mutation: 13 strains or lines available

Notes

Cell line EPD0190_3_E03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:215796

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)