Mboat7^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mboat7^{tm1a(KOMP)Wtsi}
• Name: membrane bound O-acyltransferase domain containing 7; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363399
• Synonyms: LPIAT1^-
• Gene: Mboat7 Location: Chr7:3680788-3696188 bp, - strand Genetic Position: Chr7, 2.12 cM, cytoband A1
• Alliance: Mboat7^{tm1a(KOMP)Wtsi} page
• IMPC: Mboat7 gene page

Small size and domed head in Mboat7^{tm1a(KOMP)Wtsi}/Mboat7^{tm1a(KOMP)Wtsi} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0094_4_B12, EPD0094_4_C09, EPD0094_4_C11, EPD0094_4_C12, EPD0094_4_E10, EPD0094_4_G09, EPD0094_4_G12, EPD0094_4_H10
• Germline Transmission: Earliest citation of germline transmission: J:199847
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 3691278 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 3692168. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:199847)

Targeting vector to generate the Mboat7^tm1a(KOMP)Wtsi allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Mboat7 Mutation: 19 strains or lines available

Notes

Cell line EPD0094_4_C11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:199847

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)