5031439G07Rik^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: 5031439G07Rik^{tm1e(KOMP)Wtsi}
• Name: RIKEN cDNA 5031439G07 gene; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363289
• Gene: 5031439G07Rik Location: Chr15:84828137-84872503 bp, - strand Genetic Position: Chr15, 40.25 cM
• IMPC: 5031439G07Rik gene page

Mutation origin

• Mutant Cell Lines: EPD0101_3_D02, EPD0101_3_D04, EPD0101_3_F04, EPD0101_3_G03, EPD0101_3_H01, EPD0101_3_H03, EPD0101_3_H04
• Germline Transmission: Unknown
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 84845293 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any 5031439G07Rik Mutation: 25 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)