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Targeted Allele Detail
Symbol: Crlf3tm1a(KOMP)Wtsi
Name: cytokine receptor-like factor 3; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4363171
Gene: Crlf3  Location: Chr11:80046493-80080991 bp, - strand  Genetic Position: Chr11, 47.43 cM
Mutant Cell Lines:  EPD0033_3_A11, EPD0033_3_B11, EPD0033_3_B12, EPD0033_3_C11, EPD0033_3_C12, EPD0033_3_D12, EPD0033_3_E11, EPD0033_3_F11, EPD0033_3_G11, EPD0033_3_G12, EPD0033_3_H12 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
Mutation detailsThe L1L2_gt0 cassette was inserted at position 80064889 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 80063904. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:148605, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Crlf3 Mutation:  34 strains or lines available
Cell line EPD0033_3_C11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200082
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.08
The Jackson Laboratory