D130043K22Rik^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: D130043K22Rik^{tm1a(KOMP)Wtsi}
• Name: RIKEN cDNA D130043K22 gene; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362922
• Synonyms: Kiaa0319-KO1
• Gene: D130043K22Rik Location: Chr13:25029118-25085253 bp, + strand Genetic Position: Chr13, 10.72 cM
• Alliance: D130043K22Rik^{tm1a(KOMP)Wtsi} page
• IMPC: D130043K22Rik gene page

Mutation origin

• Mutant Cell Lines: EPD0073_1_A10, EPD0073_1_A11, EPD0073_1_A12, EPD0073_1_B10, EPD0073_1_B11, EPD0073_1_B12, EPD0073_1_C10, EPD0073_1_C11, EPD0073_1_C12, EPD0073_1_D10, EPD0073_1_D11, EPD0073_1_D12, EPD0073_1_E10, EPD0073_1_E11, EPD0073_1_E12, EPD0073_1_F10, EPD0073_1_F12, EPD0073_1_G10, EPD0073_1_G11, EPD0073_1_H10, EPD0073_1_H11, EPD0073_1_H12, EPD0077_5_C04, EPD0077_5_C06, EPD0077_5_D04, EPD0077_5_D06, EPD0077_5_F04, EPD0077_5_F05, EPD0077_5_G04
• Germline Transmission: Earliest citation of germline transmission: J:242251
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25047100 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 25047964. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any D130043K22Rik Mutation: 57 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)