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Targeted Allele Detail
Symbol: Strip1tm1a(KOMP)Wtsi
Name: striatin interacting protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4362804
Synonyms: Strip1gt
Gene: Strip1  Location: Chr3:107612532-107631694 bp, - strand  Genetic Position: Chr3, 46.83 cM
Mutant Cell Lines:  EPD0316_1_A01, EPD0316_1_A02, EPD0316_1_B02, EPD0316_1_B03, EPD0316_1_C04, EPD0316_1_E04, EPD0316_1_F03, EPD0316_1_G01, EPD0316_1_G03, EPD0316_1_H03
Germline Transmission:  Earliest citation of germline transmission: J:251816
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 107625189 of Chromosome 3 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 107624163. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 2 lines available
Carrying any Strip1 Mutation:  26 strains or lines available
It is important to note that one Strip1 knockout-first cell line analyzed, EPD0316_1_A02, had a mixture of improperly targeted and correctly targeted ESC clones, based on Southern blotting, and was not used in J:251816. Cell line EPD0316_1_G03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:251816
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory