Spag8tm1(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Spag8tm1(KOMP)Wtsi |
Name: |
sperm associated antigen 8; targeted mutation 1, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4362619 |
Gene: |
Spag8 Location: Chr4:43651335-43653594 bp, - strand Genetic Position: Chr4, 23.05 cM
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IMPC: |
Spag8 gene page |
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Mutant Cell Lines: |
EPD0319_5_A02, EPD0319_5_A03, EPD0319_5_A05, EPD0319_5_C04, EPD0319_5_D04, EPD0319_5_D05, EPD0319_5_E03, EPD0319_5_E06, EPD0319_5_G03, EPD0319_5_G06, EPD0319_5_H01, EPD0319_5_H02, EPD0319_5_H03, EPD0319_5_H05, EPD0319_5_H06 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2006 starting at position 43651417 and ending at position 43651417 of Chromosome 4 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site.
(J:148605, J:173534)
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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