Rtcbtm1a(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Rtcbtm1a(KOMP)Wtsi |
Name: |
RNA 2',3'-cyclic phosphate and 5'-OH ligase; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4362526 |
Synonyms: |
Rtcbfl-laczneo |
Gene: |
Rtcb Location: Chr10:85774501-85793657 bp, - strand Genetic Position: Chr10, 41.81 cM
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Alliance: |
Rtcbtm1a(KOMP)Wtsi page
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IMPC: |
Rtcb gene page |
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Mutant Cell Lines: |
EPD0044_1_A07, EPD0044_1_A08, EPD0044_1_A09, EPD0044_1_B09, EPD0044_1_C08, EPD0044_1_F08, EPD0044_1_G08, EPD0044_1_G09, EPD0044_1_H08, EPD0044_1_H09 |
Germline Transmission: |
Earliest citation of germline transmission:
J:216740
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_gt0
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Mutation details: The L1L2_gt0 cassette was inserted at position 85786952 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 85787741. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Cell line EPD0044_1_G09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:216740
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
3 reference(s) |
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