Rnaseh2a^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnaseh2a^{tm1(KOMP)Wtsi}
• Name: ribonuclease H2, large subunit; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362394
• Gene: Rnaseh2a Location: Chr8:85683239-85694498 bp, - strand Genetic Position: Chr8, 41.38 cM, cytoband C3
• IMPC: Rnaseh2a gene page

Mutation origin

• Mutant Cell Lines: EPD0311_2_A08, EPD0311_2_C07, EPD0311_2_C09, EPD0311_2_C10, EPD0311_2_C11, EPD0311_2_D03, EPD0311_2_D05, EPD0311_2_D06, EPD0311_2_D09, EPD0311_2_E09, EPD0311_2_E11, EPD0311_2_F06, EPD0311_2_G03, EPD0311_2_G04, EPD0311_2_H08, EPD0311_2_H10
• Germline Transmission: Unknown
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 1390 starting at position 85691722 and ending at position 85693112 of Chromosome 8 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Rnaseh2a Mutation: 45 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)