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Targeted Allele Detail
Symbol: Dot1ltm1a(KOMP)Wtsi
Name: DOT1-like, histone H3 methyltransferase (S. cerevisiae); targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4362351
Synonyms: Dot1lTg
Gene: Dot1l  Location: Chr10:80755206-80795461 bp, + strand  Genetic Position: Chr10, 39.72 cM
Mutant Cell Lines:  EPD0013_1_A08, EPD0013_1_B08, EPD0013_1_C08, EPD0013_1_D08, EPD0013_1_E08, EPD0013_1_F08, EPD0013_1_G08, EPD0013_1_H08, EPD0018_2_A01, EPD0018_2_A02, EPD0018_2_A03, EPD0018_2_B02, EPD0018_2_B03, EPD0018_2_C01, EPD0018_2_C02, EPD0018_2_D01, EPD0018_2_D02, EPD0018_2_D03, EPD0018_2_E01, EPD0018_2_E02, EPD0018_2_E03, EPD0018_2_F02, EPD0018_2_F03, EPD0018_2_G02, EPD0018_2_H02, EPD0018_2_H03
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gtk
Mutation detailsThe L1L2_gtk cassette was inserted at position 80764139 of Chromosome 10 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 80764939. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 4 lines available
Carrying any Dot1l Mutation:  54 strains or lines available
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory