Nfx1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nfx1^{tm1a(KOMP)Wtsi}
• Name: nuclear transcription factor, X-box binding 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362255
• Gene: Nfx1 Location: Chr4:40970906-41025992 bp, + strand Genetic Position: Chr4, 20.72 cM, cytoband B1
• IMPC: Nfx1 gene page

Mutation origin

• Mutant Cell Lines: EPD0025_3_A10, EPD0025_3_A12, EPD0025_3_B10, EPD0025_3_B12, EPD0025_3_C10, EPD0025_3_C12, EPD0025_3_D10, EPD0025_3_D12, EPD0025_3_E10, EPD0025_3_F10, EPD0025_3_F11, EPD0025_3_F12, EPD0025_3_G10, EPD0025_3_G11, EPD0025_3_G12, EPD0025_3_H10, EPD0025_3_H11, EPD0025_3_H12
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 40988201 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 40989016. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Nfx1 Mutation: 54 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)