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Targeted Allele Detail
Symbol: Cr2tm2.1(HLA-A)Crr
Name: complement receptor 2; targeted mutation 2.1, Michael C Carroll
MGI ID: MGI:4356009
Synonyms: Cr2deltaGFP
Gene: Cr2  Location: Chr1:195136811-195176716 bp, - strand  Genetic Position: Chr1, 98.44 cM
Germline Transmission:  Earliest citation of germline transmission: J:151991
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
Mutation detailsSequence encoding the transmembrane and cytoplasmic regions of the human HLA class I alpha chain was positioned in frame adjacent to exon 15 to replace the analogous region of the endogenous gene product. A floxed neomycin selection gene and an IRES-GFP followed by a stop codon were inserted downstream before exon 16. Founder mice were crossed with cre deleter mice to remove the neomycin cassette. GFP expression was detected in B cells and follicular dendritic cells. The gene fusion product's expression levels were comparable to levels of the endogenous receptor in wild-type mice. The gene fusion product retained the ability to bind the complement factor C3d but lost the ability to form a complex with CD19. (J:151991)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cr2 Mutation:  25 strains or lines available
Original:  J:151991 Barrington RA, et al., Uncoupling CD21 and CD19 of the B-cell coreceptor. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14490-5
All:  1 reference(s)

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MGI 6.03
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