Cr2^{tm2.1(HLA-A)Crr}
Targeted Allele Detail

Summary

• Symbol: Cr2^{tm2.1(HLA-A)Crr}
• Name: complement receptor 2; targeted mutation 2.1, Michael C Carroll
• MGI ID: MGI:4356009
• Synonyms: Cr2^deltaGFP
• Gene: Cr2 Location: Chr1:194819119-194859024 bp, - strand Genetic Position: Chr1, 98.44 cM
• Alliance: Cr2^{tm2.1(HLA-A)Crr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151991
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Reporter) Mutation: Insertion Mutation details: Sequence encoding the transmembrane and cytoplasmic regions of the human HLA class I alpha chain was positioned in frame adjacent to exon 15 to replace the analogous region of the endogenous gene product. A floxed neomycin selection gene and an IRES-GFP followed by a stop codon were inserted downstream before exon 16. Founder mice were crossed with cre deleter mice to remove the neomycin cassette. GFP expression was detected in B cells and follicular dendritic cells. The gene fusion product's expression levels were comparable to levels of the endogenous receptor in wild-type mice. The gene fusion product retained the ability to bind the complement factor C3d but lost the ability to form a complex with CD19. (J:151991)

Generation of the Cr2^tm2.1(HLA-A)Crr allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cr2 Mutation: 84 strains or lines available

References

• Original: J:151991 Barrington RA, et al., Uncoupling CD21 and CD19 of the B-cell coreceptor. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14490-5
• All: 1 reference(s)