Tg(Thy1-DCTN2)M21Elfh
Transgene Detail

Summary

• Symbol: Tg(Thy1-DCTN2)M21Elfh
• Name: transgene insertion M21, Erika Holzbaur
• MGI ID: MGI:4354272
• Synonyms: Tg^dynamitin, transgenic M21
• Transgene: Tg(Thy1-DCTN2)M21Elfh Location: unknown
• Alliance: Tg(Thy1-DCTN2)M21Elfh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-DCTN2)M21Elfh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DCTN2 (10540)

  Dctn2

  (MGI:107733)

• Mutation details: The Thy1.2 promoter drives expression of a human dynamitin (DCTN2) cDNA, with a FLAG tag added to the 3' terminus of the coding sequences, in postnatal motor neurons. The M21 line was estimated to have a ~20-fold increase in dynamitin mRNA levels. Expression of the encoded protein in brain homogenates by western blot using FLAG-tag antibodies is not detected until postnatal day 13. Two additional lines (M5 and M13) were created and these had ~2- to 4-fold increased levels of dynamitin mRNA, respectively. (J:130629)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:130629 LaMonte BH, et al., Disruption of dynein/dynactin inhibits axonal transport in motor neurons causing late-onset progressive degeneration. Neuron. 2002 May 30;34(5):715-27
• All: 3 reference(s)