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Rb1tm2.1Jyjw
Targeted Allele Detail
Nomenclature
Symbol: Rb1tm2.1Jyjw
Name: RB transcriptional corepressor 1; targeted mutation 2.1, Jean YJ Wang
MGI ID: MGI:4354271
Synonyms: Rb1N750F, Rb1NF
Gene: Rb1  Location: Chr14:73194453-73325885 bp, - strand  Genetic Position: Chr14, 38.73 cM, cytoband D3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:151417
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsA targeting vector targeted to exon 22 was used to substitute a bulky phenylalanine for asparagine at amino acid 750, which is predicted to sterically block access to the LXCXE binding cleft. Southern blotting confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. (J:151417)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rb1 Mutation:  80 strains or lines available
References
Original:  J:151417 Francis SM, et al., A functional connection between pRB and transforming growth factor beta in growth inhibition and mammary gland development. Mol Cell Biol. 2009 Aug;29(16):4455-66
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/06/2019
MGI 6.14
The Jackson Laboratory