Egfl7Gt(1-13)Stmn
Gene trapped Allele Detail
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| Symbol: |
Egfl7Gt(1-13)Stmn |
| Name: |
EGF-like domain 7; gene trap 1-13, Heidi Stuhlmann |
| MGI ID: |
MGI:4353265 |
| Gene: |
Egfl7 Location: Chr2:26471095-26482694 bp, + strand Genetic Position: Chr2, 18.95 cM, cytoband B
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| Alliance: |
Egfl7Gt(1-13)Stmn page
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| Allele Type: |
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Gene trapped (Reporter) |
| Mutation: |
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Insertion of gene trap vector
Vector Type: gene trap
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Mutation details: A gene trap clone, 1-13, displayed strong alkaline phosphatase
(AP) reporter gene expression
in the extraembryonic mesoderm
and in vascular structures of
the embryo proper. Using genomic
sequences that flank the retroviral
integration site to probe an embryonic
library, a cDNA clone corresponding
to 3' sequences of Vezf1
was identified (J:53033).
It was subsequently discovered that
this clone comprised a chimeric
cDNA that contained Vezf1 sequences
at its 5' end and a second
open reading frame corresponding
to Egfl7. Further comparison of
genomic and cDNA sequences revealed
that the retroviral insertion in
the ES cell clone 1-13 had occurred
in the Egfl7 gene (F. Kuhnert
and H. Stuhlmann, unpublished
observations). This finding suggested
that the endothelial-specific expression
pattern of the AP reporter gene
observed in embryos derived from
clone 1-13 (J:53033) reflects
the expression of the endogenous
Egfl7 gene rather than Vezf1.
(J:53033, J:90268)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Egfl7 Mutation: |
17 strains or lines available
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| Original: |
J:47981 Xiong JW, et al., Large-scale screening for developmental genes in embryonic stem cells and embryoid bodies using retroviral entrapment vectors. Dev Dyn. 1998 Jun;212(2):181-97 |
| All: |
3 reference(s) |
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