Alkbh8^{Gt(RRY122)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Alkbh8^{Gt(RRY122)Byg}
• Name: alkB homolog 8, tRNA methyltransferase; gene trap RRY122, BayGenomics
• MGI ID: MGI:4350028
• Gene: Alkbh8 Location: Chr9:3335151-3391154 bp, + strand Genetic Position: Chr9, 2.02 cM, cytoband A1
• Alliance: Alkbh8^{Gt(RRY122)Byg} page

Mutation origin

• Mutant Cell Line: RRY122
• Germline Transmission: Earliest citation of germline transmission: J:238390
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: A vector containing a splice acceptor sequence upstream of a beta-geo cassette (beta-galactoside/neomycin phosphotransferase fusion) was inserted into intron 7 at chromosome position 9:33494683359589, creating a gene fusion Alkbh8-betp-geo truncated transcript. (J:238390)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRY122	CL650556 ; (MGI Seq Detail)	5' RACE	Chr9:3349467-3359588 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alkbh8 Mutation: 33 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)