Herc2^{Gt(AR0530)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Herc2^{Gt(AR0530)Wtsi}
• Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2; gene trap AR0530, Wellcome Trust Sanger Institute
• MGI ID: MGI:4346577
• Synonyms: Herc2⁵³⁰
• Gene: Herc2 Location: Chr7:55699944-55881548 bp, + strand Genetic Position: Chr7, 33.42 cM
• Alliance: Herc2^{Gt(AR0530)Wtsi} page

Mutation origin

• Mutant Cell Line: AR0530
• Germline Transmission: Earliest citation of germline transmission: J:252045
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: A gene trap vector inserted into intron 2. RT-PCR analysis of mRNA from various tissues indicated formation of a truncated mRNA fused to beta-galactosidase (beta-geo). Sequencing analysis showed fusion of beta-galactosidase after amino acid residue 24 of HERC2. Immunoblot analysis revealed that protein levels are decreased by ~50% in the brain and kidney of heterozygous mice. HERC2 activities including ubiquitin ligase and stimulation of p53 activity are reduced, as shown by increased levels of USP33 protein and lower levels of p21 mRNA in heterozygous mice. (J:252045)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AR0530	CL569810 ; (MGI Seq Detail)	5' RACE	Chr7:55700635-55700695 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

51 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Herc2 Mutation: 214 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)