Huwe1^{Gt(RRE249)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Huwe1^{Gt(RRE249)Byg}
• Name: HECT, UBA and WWE domain containing 1; gene trap RRE249, BayGenomics
• MGI ID: MGI:4128148
• Gene: Huwe1 Location: ChrX:150583803-150718413 bp, + strand Genetic Position: ChrX, 68.46 cM

Mutation origin

• Mutant Cell Line: RRE249
• Germline Transmission: Unknown
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. Trap vector is inserted into intron 4 of the gene. Immunoblot on ES cells demonstrates absence of Huwe1 protein expression. Insertion abolish the splicing of the exons that code for the essential HECT domain. (J:145660)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRE249	CC249558 ; (MGI Seq Detail)	5' RACE	ChrX:150586360-150623147 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  75 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Huwe1 Mutation: 62 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)