Nucks1^Gt(XG374)Byg
Gene trapped Allele Detail

Summary

• Symbol: Nucks1^Gt(XG374)Byg
• Name: nuclear casein kinase and cyclin-dependent kinase substrate 1; gene trap XG374, BayGenomics
• MGI ID: MGI:4125668
• Gene: Nucks1 Location: Chr1:131838272-131864059 bp, + strand Genetic Position: Chr1, 57.22 cM
• Alliance: Nucks1^Gt(XG374)Byg page

Mutation origin

• Mutant Cell Line: XG374
• Germline Transmission: Earliest citation of germline transmission: J:310028
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Not Specified)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: pGT1Lxf was inserted into intron 4 of the gene and contains the mouse En2 intron 1 sequence, an En2 exon/splice acceptor (SA) sequence, the ORF for beta-geo (a fusion gene composed of beta-galactosidase and neomycin genes) as selective maker, and a polyadenylation (pA) signal sequence. (J:310028)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG374	CC182808 ; (MGI Seq Detail)	5' RACE	Chr1:131846739-131852375 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  157 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nucks1 Mutation: 68 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)