Hgsnat^{Gt(IST11912G1)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Hgsnat^{Gt(IST11912G1)Tigm}
• Name: heparan-alpha-glucosaminide N-acetyltransferase; gene trap IST11912G1, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:4034281
• Synonyms: Hgsnat-Geo
• Gene: Hgsnat Location: Chr8:26434481-26466781 bp, - strand Genetic Position: Chr8, 14.22 cM
• Alliance: Hgsnat^{Gt(IST11912G1)Tigm} page

Mutation origin

• Mutant Cell Line: IST11912G1
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: A selectable marker beta-geo was inserted into the intron 7 of the gene leading to splicing of exon 7 into the beta-geo cassette to generate a fusion protein containing amino acid sequence encoded by exons 17 followed by aminoglycoside 3'-phosphotransferase and -galactosidase. The Btk exon in the trap construct contained termination codons in all reading frames to prevent translation of the downstream exon. Both expression of encoded mRNA measured by real-time quantitative PCR and the level of protein activity measured with fluorogenic substrate Muf--D-glucosaminide in tissues and primary cultures of skin fibroblasts from the homozygous animals were reduced to 0.61.5% of that in control mice. (J:242257)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST11912G1HMF1	EF860570 ; (MGI Seq Detail)	inverse PCR	Chr8:26452160-26452272 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Hgsnat Mutation: 39 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 8 reference(s)