HgsnatGt(IST11912G1)Tigm
Gene trapped Allele Detail
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Symbol: |
HgsnatGt(IST11912G1)Tigm |
Name: |
heparan-alpha-glucosaminide N-acetyltransferase; gene trap IST11912G1, Texas A&M Institute for Genomic Medicine |
MGI ID: |
MGI:4034281 |
Synonyms: |
Hgsnat-Geo |
Gene: |
Hgsnat Location: Chr8:26434481-26466781 bp, - strand Genetic Position: Chr8, 14.22 cM
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Alliance: |
HgsnatGt(IST11912G1)Tigm page
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Mutant Cell Line: |
IST11912G1 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
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Parent Cell Line: |
Lex3.13 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Allele Type: |
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Gene trapped (Null/knockout) |
Mutation: |
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Insertion of gene trap vector
Vector: VICTR76
Vector Type: gene trap
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Mutation details: A selectable marker beta-geo was inserted into the intron 7 of the gene leading to splicing of exon 7 into the beta-geo cassette to generate a fusion protein containing amino acid sequence encoded by exons 17 followed by aminoglycoside 3'-phosphotransferase and -galactosidase. The Btk exon in the trap construct contained termination codons in all reading frames to prevent translation of the downstream exon. Both expression of encoded mRNA measured by real-time quantitative PCR and the level of protein activity measured with fluorogenic substrate Muf--D-glucosaminide in tissues and primary cultures of skin fibroblasts from the homozygous animals were reduced to 0.61.5% of that in control mice.
(J:242257)
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Sequence Tags: |
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Sequence tag details (1 tag)
Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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Genome Context: |
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Genome Browser view of this mutation
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Original: |
J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008; |
All: |
8 reference(s) |
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