Tcirg1^tm1Ywd
Targeted Allele Detail

Summary

• Symbol: Tcirg1^tm1Ywd
• Name: T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3; targeted mutation 1, Yoh Wada
• MGI ID: MGI:3852638
• Synonyms: Tcirg1^targeted, Tcirg1^targeting
• Gene: Tcirg1 Location: Chr19:3946050-3957133 bp, - strand Genetic Position: Chr19, 3.62 cM
• Alliance: Tcirg1^tm1Ywd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150327
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: This mutant allele contains a loxP- and FRT-flanked neomycin resistance cassette, in reverse orientation, in intron 13 in addition to the EGFP coding sequence, followed by a third loxP site, inserted into the last exon. It encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. (J:150327)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcirg1 Mutation: 35 strains or lines available

References

• Original: J:150327 Sun-Wada GH, et al., Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification. J Cell Sci. 2009 Jul 15;122(Pt 14):2504-13
• All: 1 reference(s)