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Itgb1tm5.1Ref
Targeted Allele Detail
Nomenclature
Symbol: Itgb1tm5.1Ref
Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler
MGI ID: MGI:3848527
Synonyms: hpmKIlox
Gene: Itgb1  Location: Chr8:128685654-128733200 bp, + strand  Genetic Position: Chr8, 76.09 cM, cytoband E2
Hair and skin phenotypes of various keratinocyte conditional Itgb1 alleles

Show the 4 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:148885
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA 141 bp cDNA fragment of the mouse Itgb1 gene encoding for the entire cytoplasmic tail including the stop codon was inserted in frame into exon 15 and a floxed neo-tk cassette was inserted into intron 15 via homologous recombination. Cre mediated recombination then removed the neo-tk cassette leaving a single loxP site in intron 15. Analysis indicated that the mutant mRNA undergoes nonsense mediated decay. (J:148885)
Generation of the Itgb1tm5.1Ref allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Itgb1 Mutation:  13 strains or lines available
References
Original:  J:148885 Piwko-Czuchra A, et al., Beta1 integrin-mediated adhesion signalling is essential for epidermal progenitor cell expansion. PLoS One. 2009;4(5):e5488
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/19/2020
MGI 6.15
The Jackson Laboratory