About   Help   FAQ
Tg(Cela1-cre/ERT)1Lgdn
Transgene Detail
Nomenclature
Symbol: Tg(Cela1-cre/ERT)1Lgdn
Name: transgene insertion 1, Craig Logsdon
MGI ID: MGI:3848026
Synonyms: BAC-Ela-CreErT, Ela-Cre-ERT2
Transgene: Tg(Cela1-cre/ERT)1Lgdn  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsA mouse BAC (clone RP23-359O19, size 222 kb) carrying the intact elastase I (Cela1) gene was used. The BAC was modified to express CreERT. For recombination mediated targeting, arms homologous to the sequence of elastase I gene between the last exon and the polyA signal (in lower case) were added to either side of the IRES-Cre-ERT-frt-Kan-frt cassette by PCR. Using homologous recombination, the IRES-Cre-ERT-frt-Kan-frt cassette was inserted into the elastase I gene. The Kan selection marker was then removed by flp-mediated recombination. two BAC-Cela1-CreERT founders were identified by genotyping. Q-PCR based analysis indicated the presence of 2-3 copies of the transgene in BAC-Cela1-CreERT founders. (J:148748)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Cela1     Summary of all recombinase alleles driven by Cela1.
 

Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:148748 Ji B, et al., Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis. 2008 Aug;46(8):390-5
All:  15 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/30/2021
MGI 6.17
The Jackson Laboratory