Cspg4^tm1.1Trot
Targeted Allele Detail

Summary

• Symbol: Cspg4^tm1.1Trot
• Name: chondroitin sulfate proteoglycan 4; targeted mutation 1.1, Jacqueline Trotter
• MGI ID: MGI:3846720
• Synonyms: NG2^-, NG2-EYFP, NG2kiEYFP
• Gene: Cspg4 Location: Chr9:56772388-56807154 bp, + strand Genetic Position: Chr9, 30.32 cM
• Alliance: Cspg4^tm1.1Trot page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148745
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EYFP gene and floxed neo cassette were inserted into exon 1 via homologous recombination. Cre mediated recombination then removed the neo cassette. EYFP expression faithfully reflects endogenous gene expression in heterozygous mice. Western blot analysis confirmed the absence of protein expression in whole brain lysates from homozygous mice. (J:148745)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cspg4 Mutation: 100 strains or lines available

References

• Original: J:148745 Karram K, et al., NG2-expressing cells in the nervous system revealed by the NG2-EYFP-knockin mouse. Genesis. 2008 Dec;46(12):743-57
• All: 22 reference(s)