Rffl^tm1Cota
Targeted Allele Detail

Summary

• Symbol: Rffl^tm1Cota
• Name: ring finger and FYVE like domain containing protein; targeted mutation 1, Michel Cohen-Tannoudji
• MGI ID: MGI:3846097
• Synonyms: Rififylin/Carp2 knockout
• Gene: Rffl Location: Chr11:82694645-82762065 bp, - strand Genetic Position: Chr11, 50.25 cM, cytoband B5
• Alliance: Rffl^tm1Cota page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148682
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The first coding exon of the gene is interrupted by a Betageo (beta-galactosidase/neomycin resistance fusion gene) reporter/selection cassette, resulting in the in-frame joining of the Betageo coding sequence to the mouse genomic sequence encoding the first 14 amino acids of the endogenous protein. Whereas wild-type mouse embryonic fibroblasts (MEFs) express RFFL mRNA at levels easily detected by RT-PCR analysis, MEFs from embryos homozygous for this mutation produce no detectable transcript. (J:148682)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rffl Mutation: 56 strains or lines available

References

• Original: J:148682 Ahmed AU, et al., CARP2 deficiency does not alter induction of NF-kappaB by TNFalpha. Curr Biol. 2009 Jan 13;19(1):R15-7; author reply R17-9
• All: 1 reference(s)